The MyGo Pro software is capable of controlling one or more MyGo Pro instruments. This section describes the process of connecting to and configuring instruments.
Configuring Your Network
The software can connect to one or more instruments via an ethernet network. There are two ways of configuring the network to allow this:
The software and instruments are both compatible with use on a standard network, where TCP/IP addresses are assigned automatically by a DHCP server. If you do not need to configure the IP address of your PC manually, then this is likely to be the type of network you have. Connect the instrument to the network in the same way you would connect a PC, using the supplied network cable to connect to a wall socket or network switch/hub. The software will automatically connect to the instrument when it has been turned on.
The software and instruments can also be used on a local network that does NOT have automatic address assignment. The simplest direct network is where the instrument is connected directly to your PC using a single cable. It is also possible to have multiple instruments on a direct network, using a network switch/hub to connect your PC to each instrument. In either case, the configuration steps are the same.
Configuring Network Settings for a Direct Connection
Click the Start button, and select Control Panel:
Click the View network status and tasks link, in the Network and Internet section:
Click Change adapter settings in the top left:
There may be multiple adapters displayed - choose the one that you plan to connect the network cable to. This will normally be labelled “Local Area Connection” as above. Right click on the adapter icon, and select Properties from the pop-up list:
In the list in the center of the window, click to select the item called “Internet Protocol Version 4 (TCP/IPv4). Please note that this must be ”Version 4“ not ”Version 6".
Click the Properties button:
Click the Use the following IP address radio button.
In the IP address: input area, type the address “169.254.88.1”. Please ensure that you type the correct numbers in each section, separated by the dots.
Click the Subnet mask: input area, and it will be automatically filled out as “255.255.0.0”. This default value is correct and does not need to be changed.
Click the Ok button.
Click the Close button.
The network is now configured for a direct connection.
Click the System Preferences icon in the Dock:
The system preferences window is displayed, click the Network icon:
The Network settings window will be displayed. This shows all network devices in your PC. Select the “Ethernet” or “USB Ethernet” device by clicking it. If there are multiple ethernet devices, please select the one to which you will connect the network cable:
Next to Configure IPv4:, select the “Manually” option:
Now enter the IP Address: as: 169.254.88.1
Enter the Subnet Mask: as: 255.255.0.0
Please note that for both these numbers, full stops (periods) must be used between each number. Click the Apply button: The network is now configured, you may close the Network window. Second, connect your PC to a single instrument using the provided network cable, OR connect your PC and each instrument to a network switch/hub.
Finally, follow the instructions in the section on Registering a New Instrument to use the instrument with the software.
Registering A New Instrument
MyGo Pro instruments can be connected to an ethernet network, and there may be multiple instruments on your network. To use an instrument, it must be registered with the software. The software will remember your list of registered instruments, allowing you to use your preferred instruments.
Connecting An instrument To The Network
First, connect an instrument to your ethernet network, and then connect your PC to the same network. Instruments will only be automatically detected if they are present on the same subnet as your PC - this is the case on most local area networks, but please check with your network administrator if in doubt.
In the top right of the software window, click the Configuration button, to display the Configuration dialog box. By default, the About section is displayed.
Select the MyGo Pro tab to display a list of registered instruments and configuration options. If an instrument has already been registered then the software will remember it and it will appear in the Registered Instruments list.
When the software is first installed, the list will be empty, since no instruments are registered. To register an instrument, press the Add button and the Choose Instrument dialog will appear.
Adding An Instrument
The dialog now displays a list of available instruments. Any instruments that have been detected on the network, but which are not currently registered, will be displayed. If your instrument is not displayed, then check that it is powered on, connected to the same network, and displaying a green light. You may also need to press Cancel, wait for a minute or two, and then press Add again. If your instrument is displayed in the list, just click it, then click Select. (Alternatively, just double-click the instrument):
The Configuration window now displays a list of Registered Instruments as shown below. This list can be edited in the normal way, by adding, deleting and moving instruments up and down.
The new instrument will be added to the list, with a default name of “ MyGo Pro”, the appropriate serial number in brackets, and status “Checking…”. If you wait for a short time, the status will change to “Available”.
Deleting An Instrument
To delete an instrument, just select it in the list of Registered Instruments and click the Delete button. Note that this does not delete any experiments associated with the instrument. If you wish to use the instrument again later, just click Add to register it in the normal way.
Testing Instrument Connection
If an instrument’s status displays “Checking…” or “Not Connected” it is possible to test the instrument’s connection by clicking the Test Instrument Connection button. The software will attempt to connect to the instrument via the network, and to test the speed of the network:
Connection Test Succeeded
If the test is successful, the dialog below is displayed. The network data speed is displayed in megabits per second (MBit/s). The instrument requires an 8 MBit/s connection to perform reliably. This should be easily achieved by using a 100MBit ethernet network. A 10MBit ethernet connection is not supported. Click OK to close the dialog.
Connection Test Warning
If your network connection is not fast enough to run the instrument reliably, the dialog box below will be displayed:
This indicates that the instrument is connected, but you may need to contact your network administrator to resolve any network problems, and/or upgrade your network connection.
Instrument Connection Failed
If the instrument cannot be connected to, the following display will appear:
Connection Timed Out
In this case, the connection timed out while trying to connect. This indicates that the instrument is not connected to the network, or is not responding (e.g. it may not be powered).
Working with an Instrument
Click the instrument to select it (if it is not already selected), and the editor on the right hand side of the screen will display the instrument editor:
The Name: control can be used to give the instrument a new name, for example you might want to add the location and owner of the instrument. This name is displayed on your installation of the software, but other installations can use different names for the same instrument. The serial number is used to identify the instrument - the name is just provided for your convenience.
The Network Address control displays the current address used to connect to the instrument. More details on this control are given in the section on configuring the network, but on most networks there will never be any need to edit the value displayed - the instrument will be located on the network automatically.
The Serial Nr. displays the unique serial number associated with the instrument. This number is permanently associated with an instrument, and cannot be changed.
The Instrument Actions section provides several options:
Manage Dye Calibrations Files
Click Manage Dye Calibration Files to view and manage the dye calibration files stored on the instrument:
Each instrument can store a number of dye calibration files. Each dye calibration tells the instrument how to acquire data for the associated dye - in this case the instrument contains a single calibration for SYBR dye. Usually, an instrument will be calibrated for several dyes. When you execute an experiment on an instrument, the dye calibration files will be retrieved from that instrument, and used to analyse optical data.
Send A New Dye To Instrument
From time to time, new dye calibration files may be distributed, to give improved performance, or to enable the use of new dyes. Furthermore, you may wish to create your own dye file that will be covered in Automatic Dye File Generation. To load these files onto an instrument, click the Send a new dye to instrument. A dialog box will appear that will let you choose the dye file you wish to send to an instrument as shown below.
Once selected the dye file will appear in the Manage Dye Calibrations Files as shown below:
This new dye file will appear in the Dye Calibrations list under Run Settings when a new experiment has been executed on this instrument.
Save Dye Calibration From Instrument
It is also possible to retrieve dye calibration files from the instrument. Select the dye calibration file you wish to save then click the Save Dye File From Instrument button. You will be greeted with a dialog box that lets you save the dye calibration file to a specific location. This dye calibration file can then be imported into other instruments, or used in other experiments.
Saving Changes to MyGo Pro
Any dye calibration changes made to the MyGo Pro are saved when you click the Close button. The instrument will now be updated.
Retrieving Instrument Report Files
Each MyGo instrument maintains a record of performance of the instrument, which can be useful when troubleshooting the instrument. Technical support may request that you provide them with an Instrument Report File from the instrument, when resolving an issue.
Select an instrument, and click Retrieve Instrument Report File. You will be asked to select a location for this report file as follows:
You will need to wait while the software retrieves the report file from the instrument:
Once retrieved you will be presented by the following dialog box which indicates that the instrument report file has been successfully retrieved.
Updating Your Instrument via the Network
From time to time, new software may be released. In some cases, the software will include improvements for the MyGo Pro, which will require the instrument to be updated. When this happens the instrument’s status will read Needs update when adding an instrument in the Choose instrument window as show below:
When this machine has been added to the Registered Instruments list the same status will be present as shown below:
Alternatively you may not be able to connect to the instrument as it requires updating. If this is the case the following dialog box will appear:
If you attempt to start a run without updating the Experiment Status Bar will display the following message: Instrument Connection Error, as shown below.
To investigate the connection error select Show log… An error message in the Details box indicates an update is required.
You will be prompted to perform the update when starting a run. A dialog will be displayed as follows:
After reading and following the instructions, click the “Update Firmware” button. The update procedure will take a few minutes, during which time a dialog will be displayed:
When the procedure is complete, a dialog will inform you that you need to restart the instrument:
To do this disconnect the power cable from the instrument, then reconnect it. The instrument will now start up as normal, with the upgrade complete. You can now proceed with your run.
In the event that the instrument is disconnected from the network, or loses power during the upgrade procedure, the instrument will need to be restarted. Disconnect the power cable from the instrument, then reconnect it. The instrument will now start up in a special mode. You should now attempt to start your run again. You will be prompted to update the firmware again. Continue as before, ensuring that the instrument remains connected to the network and power during the entire upgrade procedure.